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          Adipogen公司產品JC-1簡介

          2022-05-31
          瀏覽次數: 25

          Adipogen公司產品JC-1簡介

          產品名稱:JC-1

          別名:5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide

          產品描述:The membrane-permeant dual-emission potential-sensitive JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria. JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. After cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to other commonly employed fluorescent probes of mitochondrial membrane potential is that it allows qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio.

          保存溫度:-20°C

          運輸溫度:Ambient

          預期分子量:652.2

          產品形式:liquid

          有效期:Stable for at least 2 years after receipt when stored at -20°C.

          產地:瑞士

          生產商:AdipoGen Life Sciences

          CAS號:3520-43-2 and 47729-63-5

          分子式:C25H27Cl4IN4

          溶解度:Soluble in DMSO, ethanol, methanol or acetone.


          文獻引用:

          1.J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential: M. Reers, et al.; Biochemistry 30, 4480 (1991)

          2.Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1: S.T. Smiley, et al.; PNAS 88, 3671 (1991)

          3.A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1): A. Cossarizza, et al.; BBRC 197, 40 (1993)

          4.Mitochondrial membrane potential monitored by JC-1 dye: M. Reers, et al.; Methods Enzymol. 260, 406 (1995)

          5.JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis: S. Salvioli, et al.; FEBS Lett. 411, 77 (1997)


          訂購詳情:

          品牌產品名稱產品貨號規格
          AdipogenJC-1

          AG-CR1-3568-M001

          AG-CR1-3568-M005

          AG-CR1-3568-5001

          1mg

          5mg

          5 x 1 mg


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